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耗材專區 | Empore™ E係（xì）列技術簡介

更新時間：2025-01-22 點擊次數：2559

目前蛋（dàn）白質組（zǔ）學耗材成本高昂。

現有蛋白質組學方法需要很高的技術（shù）門檻。

需要建立簡單（dān）易用和隨時可用的（de）方法。

Empore™ E係列技術簡介

E3™技（jì）術特點

高效：手動操作（zuò）時（shí）間<15min；
穩定：與多種細胞裂解條件兼容（例如：尿素、SDS、RIPA、TFA等）；
經濟：彌補基因組學和蛋（dàn）白質組學產品之間的（de）差距；
易用：對入門級生（shēng）物醫學工作者零技術障礙；
多樣：E3tips™、E3filter™和E3plate™可滿足不同的樣品體積、濃度、數量和（hé）自動化的需求；
簡便：無（wú）需（xū）液體轉（zhuǎn）移，無需考慮任何與遊離磁（cí）珠相關的問題（蛋白與磁珠的比例、磁珠粘附（fù）在管壁和表麵、交（jiāo）叉汙染等）；
Empore™產（chǎn）品服務於蛋白組學領域20多年，有深厚的應用基礎。

E3™產品類型

E3filter測試結（jié）果

圖（tú）片

Fig.1 Qualitative and quantitative assessment of E3technology (E3 filter) for E. coli proteome analysis. (A-C) Comparison of the number of proteins, peptides, and PSMs between the E3filter, FASP, and SP4-GB approaches. Error bars represent three replicates. (D-E) Overlapping analyses of proteins and peptides derived from the three methods. (F) Coefficient of variation of quantified proteins by the three methods. (G) Coefficient of variation of quantified peptides. (H) Pearson correlation between replicate experiments and different methods. (I) Percentages of missed cleavages. (J-K) Volcano plot of showing significantly differential proteins between the three methods. The two curves show FDR 0.05 and 0.01, respectively. (L) Heatmap of the overall quantified proteins by the three methods. FASP, filter aided sample preparation; SP4, Solvent Precipitation SP3; GB, glassbeads.

E3工作流程

E4工作（zuò）流（liú）程（chéng）

參（cān）考文獻

Yu YB, et al. Cell Reports Methods 4, 2024, 100796, June 17.
Johnston HE, et al.Anal Chem.2022, 94:10320-10328.
WiśniewskiJR, et al. Nat Methods. 2009,6:359-62.